Transcriptional profiling of conditional knockout mice lacking Izumo1r in FoxP3+ Tregulatory cells

We reported transcriptional characterization of splenic, ear skin and back skin of Izumo1R flox X Foxp3Cre mice and littermate controls. We report few changes observed in splenic Tregs but changes seen in the whole skin transcriptome and gamma delta compartment starting at approximately 8 weeks of age. We report upregulation of inflammatory genes overlapping with genes upregulated in the skin following topical administration of Imiquimod. Overall design: For the IMQ treated samples, dorsal and ventral sides of the ear were treated with 125ug of 5% Imiquimod cream until indicated date. All other data are from untreated mice at indicated age. Foxp3/GFP+ Treg cells were sorted and processed for RNA-Seq. TCRB+ CD4+ Foxp3GFP- cells were sorted from the skin as Tcon. TCRb-CD19-GammaDeltaTCR+ cells from the skin were sorted as skin gamma delta T cells. All sorted samples were pre-gated on DAPI- single cells.