Sema3A relieves Neuropathic Pain by Reducing eIF2a Phosphorylation via Suppressing PI3K Akt mTOR Pathway

Ribo-seq, which a method that sequences RNA fragments being translated by ribosomes, enables accurate quantification of all translatable molecules in a sample, bridging the gap between the transcriptome and proteome. PKR inhibitor can inhibit eIF2a phosphorylation and we have proved in our article that PKRi treated can relieve neuropathic pain. Here, we used Ribo-seq to explore the effects of different various treatments on the translation profile of mice DRG, we performed Ribo-seq on DRG from three groups: sham, CCI 14th day, and CCI+PKRi inhibitor groups. In order to explore the downstream of eIF2a.