Multimodal single-cell profiling reveals crosstalk between macrophages and stromal cells in poor prognostic cholangiocarcinoma patients

Here, we performed single nuclei sequencing in 7 perihilar cholangiocarcinoma (pCCA) patients. Patients were divided into 2 clinical outcomes based on clinical data. We performed cell-cell interaction (CCI) network analysis to identify unique ligand-receptor pairs within the poor clinical outcome group. Later we validated 3 of those pairs using multiplexed immunohistochemistry. In addition, we used high dimensional cytometry to examine the immune phenotype of 15 CCA patients.