Homo sapiens Raw sequence reads

The acetylation modification of lysine 9 on histone H3 and lysine 27 on histone H3 is an important mechanism of transcriptional regulation. In this study, CUT&TAG sequencing was performed on cell samples of the HuCCT1 cell line with targeted shRNA against the SLC16A3 gene sequence and on empty vector controls, with the aim of analyzing the levels of H3K9ac and H3K27ac across the genome. The results showed that the knockdown of SLC16A3 expression resulted in a significant increase in both H3K9ac and H3K27ac levels across a wide range of genes, leading to an upregulation of acetylation levels at TSS regions for a series of important tumor suppressor genes. Additionally, transcriptome sequencing was conducted on these same samples, and its results were compared and intersected with those from CUT&TAG to verify that the upregulation in acetylation levels at TSS regions corresponded to an increase in expression level for these tumor suppressor genes at the transcriptional level.