Data from: Destabilization of mutualistic interactions shapes the early heat stress response of the coral holobiont

Background: This study explores the stability of the symbiotic relationship between coral and their dinoflagellate algae (Symbiodiniaceae) under increasing heat stress. In a 2-month manipulative experiment, the thermotolerant coral Porites lutea was exposed to increasing temperatures, from 28˚C to 32˚C (0 – 8 Degree Heating Weeks, DHW), and the stability of the host-symbiont relationship was assessed through coral and Symbiodiniaceae transcriptomics, microbial 16S rRNA gene sequencing and physiological measurements. The data record contains: link to the NCBI BioProject containing raw sequence data generated from 16S rRNA gene sequencing (PRJNA1050834) link to the NCBI BioProject containing raw sequence data generated from mRNA sequencing (PRJNA1050834) one csv file with the coral Health score used in the analyses one csv file with the photosynthesis and respiration data one csv file with the Symbiodiniaceae photochemical effective efficiency data link to the GitHub repository with the R scripts generated for the analyses Software/equipment used to create/collect the data: 16S rRNA amplification (515F-806rB primers) of gDNA from coral (Porites lutea) and seawater samples; Illumina MiSeq sequencing (2x250 bp). RNA sequencing from coral samples; NovaSeq 6000 S2 (2 x 100 bp) health score assessment over time using the CoralWatch Coral Health Chart (Siebeck et al 2006) measures of net photosynthesis and respiration rates over time by assessing oxygen concentration using an Optical probe after placing corals in 600 mL plastic chambers for 50-min incubation under light conditions (130 µmol photons m−2 s−1) and 75-min incubation in dark conditions, respectively. measures of photochemical effective efficiency over time using a mini-PAM fluorometer (Walz, Germany; settings: MI = 4; SI = 8; SW = 0.8; G=2; D=2) Software/equipment used to manipulate/analyse the data: amplicon paired-end reads were analysed in QIIME 2 (v2020.8), and output data were imported and analysed in R (v 4.0.3). reference-based transcirptomics was applied using the RNA-seq aligner STAR, and output data were imported and analysed in R (v 4.0.3). physiological measures were analysed in R (v 4.0.3).研究背景：本研究探讨了升温胁迫下珊瑚与其共生的虫黄藻（Symbiodiniaceae）之间的共生关系稳定性。本研究通过一项为期2个月的操控性实验，将耐热性珊瑚团块滨珊瑚（Porites lutea）置于从28℃升至32℃的升温梯度下（对应0~8个热周（Degree Heating Weeks, DHW）），并通过珊瑚及虫黄藻转录组学（transcriptomics）、微生物16S rRNA基因测序与生理指标测定，评估宿主-共生体的共生关系稳定性。 本数据集包含以下内容： 1. 包含16S rRNA基因测序原始序列数据的NCBI BioProject链接（编号：PRJNA1050834） 2. 包含mRNA测序原始序列数据的NCBI BioProject链接（编号：PRJNA1050834） 3. 一份用于本研究分析的珊瑚健康评分CSV文件 4. 一份包含光合作用与呼吸作用数据的CSV文件 5. 一份包含虫黄藻光化学有效效率数据的CSV文件 6. 包含本研究分析所用R脚本的GitHub仓库链接 数据采集与生成所用软件及设备： 1. 针对珊瑚（团块滨珊瑚Porites lutea）与海水样本的基因组DNA进行16S rRNA扩增（引物为515F-806rB），并采用Illumina MiSeq测序平台进行双端250 bp测序 2. 珊瑚样本的RNA测序采用NovaSeq 6000 S2测序平台（双端100 bp测序模式） 3. 采用CoralWatch珊瑚健康评分表（Siebeck等，2006）进行随时间变化的珊瑚健康评分评估 4. 通过将珊瑚置于600 mL塑料培养箱中，分别在光照条件（光照强度130 μmol光子·m⁻²·s⁻¹）下孵育50分钟、黑暗条件下孵育75分钟后，利用光学探针检测氧浓度，以测定随时间变化的净光合速率与呼吸速率 5. 采用mini-PAM荧光光度计（德国Walz公司；仪器参数设置：MI=4、SI=8、SW=0.8、G=2、D=2）测定随时间变化的光化学有效效率 数据处理与分析所用软件及设备： 1. 扩增子双端测序读段在QIIME 2（v2020.8）中进行分析，所得结果导入R（v4.0.3）中进行后续处理与统计分析 2. 采用RNA-seq比对工具STAR进行基于参考基因组的转录组分析，所得结果导入R（v4.0.3）中进行后续处理与统计分析 3. 生理指标数据在R（v4.0.3）中进行分析