Purification of Recombinant Cas9
收藏NIAID Data Ecosystem2026-05-02 收录
下载链接：
https://zenodo.org/record/1325936
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资源简介：
SGC Open Notebook Project to Characterize the HMTase NSD3
Exp027 Objective: To purify recombinant Cas9 protein for genome editing experiments. Recombinant Cas9 will be used with synthesized crRNA:tracrRNA to assemble ribonucleoproteins (RNPs) to target the nuclease activity of Cas9 to specific loci within the genome. Using RNPs as opposed to viral delivery of CRISPR gene editing components is advantageous as the expression of Cas9 is transient limiting off-target effects.
创建时间：
2024-08-02
