Quantifying protein aggregation in Drosophila Clone 8 cells co-transfected with GCN4-Ci (a predominantly-aggregated protein) and eGFP (a predominantly-soluble protein) in various ratios.

Panel A: Sample images from the five constructs tested. Panel B: Western blot showing the results of subcellular fractionation of the five constructs. Panel C: Quantification of the western blot in panel B using ImageJ. Panel D: values for the five constructs tested. The number of cells analyzed in constructs 1, 2, 3, 4 and 5 is 17, 15, 16, 27 and 20 cells, respectively. Error bars represent the standard error of the mean (SEM).