Effect of the AMPK inhibition by compound C in spermatozoa viability.

Spermatozoa were incubated in TBM or TCM medium at 17°C or 38.5°C in absence or presence of the AMPK inhibitor CC (30 µM) for different times (1–24 h). Spermatozoa viability was measured by flow cytometry using SYBR-14 and IP as probes. This experiment was performed at least 4 times and the results expressed as percentage of viable cells are shown as mean ± standard error of the mean. Statistical differences are shown as a,b when p