qRT-PCR Ct values and relative titers of cyst nematode viruses within plant parasitic nematode (PPN).

Mean qRT-PCR cycle threshold (Ct) values are shown for soybean cyst nematode (SCN) nyami-like virus (NLV), SCN bunya-like virus (BLV), potato cyst nematode (PCN) picorna-like virus (PLV), and internal PPN control (18S). Relative viral titers were calculated and log2 adjusted by comparison against internal control using a modified Pfaffl method [7,26,27]. Negative log2 relative titers denote a value below the internal control; whereas, positive values represent titers higher than the internal controls. ND (not detected) demonstrates that virus was not found in the sample. Experiments were conducted in technical triplicates and standard deviations are shown. Abbreviations: J2 (second-stage juvenile), CU (Cornell University), MU (University of Missouri), NCSU (North Carolina State University).