RNA-sequencing of testes from control and Hormad1&2SFB/SFB mice

To examine whether Hormad1&2SFB/SFB (dsfb) male mice has defect in the meiotic sex chromosome inactivation (MSCI) process or not, three pairs of P19 testes were collected from each strain and mRNA-sequencing was performed. The data revealed that the transcription of X and Y chromosomes, but not autosomes, were specifically elevated. Therefore, MSCI was defective in dsfb spermatocytes. Overall design: Three pairs of PD19 testes from different mice were isolated and mRNA-sequencing was performed on the samples.