iValiD-TB: A fully characterized Mycobacterium tuberculosis dataset for antimicrobial resistance bioinformatics workflow validations

For clinical assay validations, well-characterized samples are essential for assessing methodology sensitivity and specificity. To support the community in the development of clinical next-generation sequencing assays for Mycobacterium tuberculosis, we released a comprehensive dataset of 50 whole genome sequences from characterized strains, complete with drug susceptibility and mutation profiles., , , # iValiD-TB: A fully characterized Mycobacterium tuberculosis dataset for antimicrobial resistance bioinformatics workflow validations [https://doi.org/10.5061/dryad.4j0zpc8m8](https://doi.org/10.5061/dryad.4j0zpc8m8) We have assembled a comprehensive dataset of 50 well-characterized whole genome sequences (WGS) from *Mycobacterium tuberculosis* strains to aid in the development of clinical assays for this pathogen. This dataset includes complete whole genome sequences paired-end read sets obtained through Illumina MiSeq sequencing, along with detailed profiles of drug susceptibility patterns and mutations known to be associated with antimicrobial resistance (AR) to nine MTB drugs. This dataset has been curated to be inclusive of a broad range of lineage diversity, drug susceptibility profiles, and mutation types. The sequence reads dataset has been made available at the NCBI Read Archive under Bioproject PRJNA980174. ## Description of the data and file structure The metadata, resis...在临床检测方法验证工作中，经过充分表征的样本是评估方法灵敏度与特异性的必要前提。为助力学界开发针对结核分枝杆菌（*Mycobacterium tuberculosis*）的临床下一代测序检测方法，我们发布了一套涵盖50株已充分表征菌株的全基因组序列综合数据集，并同步提供完整的药物敏感性与突变谱信息。 # iValiD-TB：一款用于抗菌耐药性生物信息学流程验证的全表征结核分枝杆菌数据集 [https://doi.org/10.5061/dryad.4j0zpc8m8](https://doi.org/10.5061/dryad.4j0zpc8m8) 我们整合了一套包含50株已充分表征的结核分枝杆菌全基因组序列（WGS）的综合数据集，以支持针对该病原菌的临床检测方法开发。本数据集涵盖通过Illumina MiSeq测序获得的完整全基因组双端读段数据集，同时附带针对9种结核分枝杆菌（MTB）药物的抗菌耐药性（AR）相关药物敏感性谱与已知突变的详细信息。该数据集经过精心整理与质控，涵盖了广泛的谱系多样性、药物敏感性谱与突变类型。序列读段数据集已上传至NCBI读段归档库（NCBI Read Archive），对应生物项目编号为PRJNA980174。 数据与文件结构说明 元数据、耐药性[原文截断]