Transcellular chaperone signaling is an intercellular stress-response that is distinct from the HSF-1 mediated HSR [RNA-seq]

The goal of this RNA-Seq analysis was to identify genes differentially expressed in a C. elegans strain expressing a neuron-specific or gut-specific hsp-90 hairpin RNAi construct compared to control animals of the same genetic background (strain AM994). Tissue-specific expression of an hsp-90 hairpin RNAi construct knocks down hsp-90 in a tissue-specific manner and induces transcellular chaperone signalling that enhances organismal proteostasis. This study aimed to identify components of the signalling pathway responsible for this effect. Gene expression profile of Day 1 adults C. elegans expression a gut-specific (hsp-90int) or neuron-specific (hsp-90neuro) hsp-90 hairpin RNAi construct compared to control animals harboring the same genetic background (strain AM994) and wild type (N2 Bristol), grown at 20C. 3 replicates of each sample.