Infection and immune status of RT-SHIV-challenged macaques.

ˆCD4 counts taken on day 84 for DE38, day 189 for FI76, FI78, FI70, FI71, FI73, and FI75, and day 196 for FI86 and FI87. $Co-cultures of PBMCs with 174×CEM cells were scored as positive by microscopic examination of syncytia formation and in some instances verified by PCR for SIV gag. &DE38 had two timepoints of positive IFNγ responses at weeks 8 and 10 post challenge. *GF14 - average 715 copies/ml on day 42 post challenge, but the animal remained below the level of detection at all other time points. %500 µM of MIV-150 in MC administered once 30 min prior to challenge (1X) or 24 h before, 30 min before, and 30 min after virus challenge (3X). @These animals exhibited low-level viremia but ultimately controlled infection to undetectable levels even after CD8 depletion. Ab positivity was defined as having at least two positive OD values above baseline at 4 weeks post challenge and IFNγ positivity was defined as having at least 50 SIV-specific IFNγ SFCs per 106 PBMCs on more than one time point post challenge. ND, not determined.