Comprehensive analysis of microRNA after moderate intensity exercise in mice

Moderate exercise is important for health; however, individuals differ in moderate intensity and it is difficult to identify. The purpose of this study was to identify new objective indicators to determine effective exercise intensity. The mice were randomly divided into the three groups: normal caged mouse group (control group, n = 3), aerobic exercise group (AE group, n = 3), and resistance exercise group (RE group, n = 3). The mice in AE group and RE group were exercised in each effective intensity and blood samples were obtained. Total RNA was isolated and analyzed using 3D-Gene miRNA Labeling kit and 3D-gene Human miRNA Oligo Chip (Toray Industries, Inc., Kanagawa, Japan).