Expression data to the study: Long non-coding RNAs in hypoxia and oxidative stress - novel insights investigating a piglet model of perinatal asphyxia.

A total of 42 newborn piglets were randomized into four study arms: 1. hypoxia-normoxic reoxygenation, 2. hypoxia-3 minutes of hyperoxic reoxygenation, 3. hypoxia-30 minutes of hyperoxic reoxygenation, and 4. sham-operated controls. Expression of the lncRNAs BDNF-AS, H19, MALAT1, ANRIL, TUG1, PANDA and related target genes VEGFA, BDNF, TP53, HIF1alpha, and TNFalpha were assessed in cortex, hippocampus, white matter, and cerebellum by qPCR and Droplet Digital PCR (ddPCR). The overall aim of this study was to explore the lncRNAs expression changes involved in the regulation of the oxidative stress response in perinatal asphyxia using a piglet model. Our results of different brain regions exhibited that the exposure to hypoxia causes significant alteration of expression in various lncRNAs, mainly in the cortex and hippocampus. Even a short exposure to 100% oxygen for 3 minutes caused expression changes in the lncRNAs, providing further evidence for oxidative brain injury caused by hyperoxic reoxygenation in newborns.本研究共纳入42头新生仔猪，随机分为4个研究组别：1. 低氧-常氧复氧组，2. 低氧-高氧复氧3分钟组，3. 低氧-高氧复氧30分钟组，4. 假手术对照组。采用实时定量聚合酶链反应（qPCR）与液滴数字聚合酶链反应（ddPCR），检测了皮层、海马体、白质及小脑中长链非编码RNA（lncRNAs）BDNF-AS、H19、MALAT1、ANRIL、TUG1、PANDA及其相关靶基因VEGFA、BDNF、TP53、HIF1α、TNFα的表达水平。 本研究以仔猪为实验模型，旨在探究围产期窒息氧化应激应答调控过程中涉及的长链非编码RNA表达变化。针对不同脑区的分析结果显示，低氧暴露可导致多种长链非编码RNA的表达发生显著改变，该效应在皮层与海马体中尤为突出。即便仅将仔猪短时间暴露于100%氧气环境中3分钟，也会引发长链非编码RNA的表达变化，这为新生儿高氧复氧所致的氧化性脑损伤提供了进一步的实验佐证。