Elucidating the Multi-Target Mechanisms of Danggui Biejia Decoction Against Hepatic Fibrosis: An Integrated Network Pharmacology and Experimental Study

This dataset contains the raw experimental data, bioinformatics datasets, and supplementary materials supporting the manuscript titled "Elucidating the Multi-Target Mechanisms of Danggui Biejia Decoction Against Hepatic Fibrosis: An Integrated Network Pharmacology and Experimental Study". The data provides a comprehensive foundation for the network pharmacology screening and the in vitro validations presented in the study. The dataset is organized into the following main components: 1. Western Blot Images: Western Bot images evaluating the expression and phosphorylation of key proteins (including Smad2, AKT, NF-κB p65, TNF-α, COL1A1, α-SMA, and β-actin) in LX-2 cells, providing raw validation for the TGF-β/Smad, PI3K/AKT, and NF-κB signaling pathways. 2. Active Components and Targets: A comprehensive dataset detailing the active ingredients identified in the five constituent herbs of Danggui Biejia Decoction (DGBJD). This includes key pharmacokinetic parameters such as Oral Bioavailability (OB) and Drug-Likeness (DL) values, alongside their predicted corresponding protein targets. 3. Disease Targets Database: The complete set of hepatic fibrosis-related targets retrieved and consolidated from public databases (GeneCards, OMIM, and CTD), which was utilized for disease-drug intersection and network construction. 4. Supplementary Tables: Three additional supplementary tables containing detailed downstream analytical results (such as topological parameters, GO/KEGG enrichment details, or molecular docking scores) that support the manuscript's findings.本数据集包含支持题为《当归鳖甲汤抗肝纤维化的多靶点机制解析：整合网络药理学与实验研究》的手稿所需的原始实验数据、生物信息学数据集及补充材料。本数据集为该研究中开展的网络药理学筛选与体外验证工作提供了全面的研究基础。 本数据集主要包含以下核心组成部分： 1. 蛋白质免疫印迹（Western Blot）图像：用于评估LX-2细胞中关键蛋白（包括Smad2、AKT、NF-κB p65、TNF-α、COL1A1、α-SMA及β-actin）的表达与磷酸化水平，为TGF-β/Smad、PI3K/AKT及NF-κB信号通路的验证提供原始实验依据。 2. 活性成分与靶点数据集：详细收录了当归鳖甲汤（Danggui Biejia Decoction, DGBJD）五味组成药材中鉴定得到的活性成分信息，涵盖口服生物利用度（Oral Bioavailability, OB）、类药性（Drug-Likeness, DL）等关键药代动力学参数，以及其预测对应的蛋白靶点。 3. 疾病靶点数据库：从公共数据库（GeneCards、OMIM及CTD）中检索并整合得到的全套肝纤维化相关靶点，用于开展药物-疾病靶点交集分析与调控网络构建。 4. 补充表格：包含3张附加补充表格，收录了支撑手稿研究结论的详细下游分析结果（如拓扑参数、基因本体（Gene Ontology, GO）/京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析详情，或分子对接评分等）。