Role of mechanometabolism in hematopoietic stem cell specification

The mechanical force generated by blood flow stimulates emergence of the first hematopoietic stem cells (HSCs) in the embryo that are critical for producing the adult blood system. Fluid force drives transition of HSC precursors from an endothelial to hematopoietic identity. Here, we provide the source data for our study exploring the molecular regulation of this fate switch. We identify a role for shear stress in driving the adaptation of mitochondrial composition, ultrastructure, and function, which we show are essential for hematopoietic fate specification and engraftment potential. Data in support of characterization of Ncx1 cardiac mutants and ex vivo shear stress cultures are supplied for all graphs in our study. Briefly, we show that shear stress remodels mitochondria by promoting mitochondrial gene transcription and protein synthesis. Laminar fluid flow selectively initiates translation of 5’terminal polypyrimidine (5’TOP) motif-containing transcripts, which commonly encode ribo..., The source data provided are derived from a comprehensive collection of methods for analysis of gene expression, hematopoietic and endothelial cell type classification, mitochondrial and bioenergetic features, protein synthesis and regulatory sequences that dictate translation of specific mRNA transcripts, hematopoietic progenitor and stem cell function, intracellular signaling pathways, and tissue concentration of drugs in the embryo following in utero administration to mothers. Detailed methods are provided in the published work associated with this study.
, # Data from: Role of mechanometabolism in hematopoietic stem cell specification

Dataset DOI: [10.5061/dryad.b5mkkwhss](https://doi.org/10.5061/dryad.b5mkkwhss)

## Description of the data and file structure

Data are stored in comma-separated value (.csv) format. A total of twenty-six (26) files are included.

Each .csv file is named according to the corresponding figure number and panel in the published study associated with the data. For example, the file 1C.csv corresponds with raw data for Figure 1C.

**Description:** The data were generated by several methods for analysis of gene expression, hematopoietic and endothelial cell type classification, mitochondrial and bioenergetic features, protein synthesis and regulatory sequences that dictate translation of specific mRNA transcripts, hematopoietic progenitor and stem cell function, intracellular signaling pathways, and tissue concentration of drugs in the embryo following in utero administration to mothers. The raw or normalized nu...,