Expression data from rat bone marrow mesenchymal stem cells

Mesenchymal stem cells (MSCs) are involved in tissue homeostasis and repair, and can be impaired in subjects with high fat diet (HFD)-induced metabolic disorders. We used microarrays to detail the global programme of gene expression bone marrow MSCs (BMSCs) underlying HFD-induced progression of metabolic disorders in rat and identified distinct classes of differentiatilly expressed genes during this process. Six-week old Sprague-Dawley (SD) rats were randomized to normal diet (ND) and high-fat diet (HFD) groups, fed a normal diet (ND, 100% basal diet) and a customized high-fat diet (60% basal diet, 10% sucrose, 20% lard and 10% egg yolk powder), respectively. The total mRNA of BMSCs (P1) from both groups (n= 3 per group) at the specified feeding time points (1, 2 and 4 months) was purified and hybridized on Affymetrix microarrays. BMSCs were isolated and characterized for the expression of cell surface markers before RNA extraction, to improve the accuracy of expression profiles.