Characterization of mouse tissues by bulk RNA sequencing

Total RNA of mice tissues (liver, small intestine, lung and kidney) was extracted using liquid N2 and Trizol. mRNA extracted from total RNA was subjected to double-stranded cDNA synthesis and Illumina stranded paired end library prep for sequencing. Liver, small intestine, lung and kidney mRNA profiles of 6-10 week old mice were generated by RNA sequencing.采用液氮与Trizol提取小鼠组织（肝脏、小肠、肺脏及肾脏）的总RNA。从总RNA中纯化得到的mRNA经双链cDNA合成后，构建Illumina链特异性配对末端测序文库用于测序。本数据集通过RNA测序获取了6至10周龄小鼠的肝脏、小肠、肺脏及肾脏的mRNA表达谱。