Figure 7

BMK-DKO cells expressing CMcl-1, transfected with VBH3 proteins treated with BH3 mimetics. Data displayed in Figure 7a-d was generated from combining results of 3 screens: 1) mim1_Mcl1_screen {Rep1_20180906, Rep2_20180907, Rep3_20180908, Rep4_20180909} 2) mim2_Mcl1_screen {Rep1_20190408, Rep2_20190410, Rep3_20190411, Rep4_20190412} 3) Mcl1_ABT199_screen {Rep1_20191024, Rep2_20191025, Rep3_20191026, Rep4_20191027} Figure 7e-f was generated from BH3 swap screen {Rep1_20200821, Rep2_20200827, Rep3_20200902, Rep4_20200904} -For each screen, see "Master Platemap" for screen design as well as data generated in qF3 analysis step 8_CombineBinnedReps: Here are, "combined_WellID_binnedReps_.csv" data files, that were fit to determine live cell Kds. Exported result from fitting these data is also included in, "CombinedBinnedReps_Results.xlsx". -Figure 7a-c shows the average of controls (VBH3 proteins and corresponding collisional control: VBH3-4E) included in each of these screens. (See "Summarized_CMcl1_ Results.xlsx").