Differential dynamic plasma miRNA profiles during the peri-implantation period in patients with different reproductive outcomes following embryo transfer

To investigate the differential dynamic plasma miRNA profiles during the peri-implantation period in patients with different reproductive outcomes following embryo transfer. 10 negative pregnancy (NP) patients, 10 biochemical pregnancy loss (BPL) patients, and 10 clinical pregnancy (CP) patients were enrolled for miRNA-seq in this study. Peripheral blood samples were collected at three time-points (ET0, ET11, and ET14) from BPL patients and CP patients, and samples were collected at two time-points (ET0 and ET11) from NP patients. Small RNA libraries were constructed using peripheral plasma RNA. miRNA-seq was used to investigate the differential dynamic peripheral miRNA changes between the three groups during the peri-implantation period. We prospectively recruited a cohort undergoing embryo transfer (ET). Peripheral llood samples were collected on ET day before ET (ET0), 11 days after ET (ET11), and 14 days (ET14) after ET. The patients were divided into the negative prengancy (NP), biochemical pregnancy loss (BPL), and clinical pregnancy (CP) groups according to serum hCG levels. Ten patients in each group were randomly selected for miRNA-seq.