Effect of Sirt6 knockout on gene expression in P7 neural stem cells

As a member of class III histone deacetylases, Sirt6 has various functions including regulation of genomic stability, DNA repair, cancer, metabolism and ageing.Deficiency of Sirt6 is lethal and causes brain development redardation. We generated Emx1-cre induced Sirt6 conditional knockout mice model and found that Sirt6 deficiency promoted self-renew of neural precursor cells (NPCs) and inhibited differentiation. This result promotes understanding of the role of Sirt6 in brain development and NPCs’ fate determination and provides clues to explain the generality and difference between species. To investigate the influence of Sirt6 on neural stem cells, we isolated neural progenitor cells from WT and Sirt6 cKO P7 mice and cultured in DMEM/F12 proliferation medium.For neurosphere RNA-seq, RNA was isolated from the 7th day culturing NPCs according to the manufacturer’s instructions. RNA-seq was performed by Annoroad, China. Data analysis was performed by Active Motif and Novogene.