Research on the Effects of Angelica Dahurica Radix on Migraine Serum metabolomics 1

Serum samples from each group were collected in metabolic cages and stored in a -80 ℃ refrigerator until analysis. Four times the volume of cold methanol was added to the precisely weighed samples and homogenized thoroughly. The mixture was then subjected to ultrasonic treatment in an ice bath for 20 min. Subsequently, the supernatant was centrifuged at 13,000 rpm for 10 minutes at 4 ℃. Metabolomic analysis was performed using a ThermoFisher UHPLC(ThermoFisher Scientific, MA, USA)) system coupled with an Orbitrap mass spectrometer. For both Electrospray ion source (ESI) positive and negative modes, the mobile phases consisted of A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile), with a flow rate of 0.3 mL/min.The study protocol received ethical approval (2019-08) from Jthe Ethics Committee of Chengdu University of Traditional Chinese Medicine, with all procedures conducted in strict accordance with established animal welfare standards.