Figure.S2.Jurkat
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http://flowrepository.org/id/FR-FCM-ZZUU
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To extend the capabilities of SLA to include transcription factors other than NFkB. In this instance, we use NFAT.
Conclusion:
PMA/Ionomycin stimulation leads to detectable nuclear import of NFAT in Jurkat cells. All antibodies used in this experiment were rigorously titrated. CD45:Histone H3 interaction was our negative control, and served as baseline for background signal. dsDNA:Histone H3 was our positive control, serving as the upper limit for detection. We used both of these controls to benchmark the NFAT:dsDNA interaction.
创建时间：
2017-01-01
