Gene expression data from wildtype and Grin1 deleted bone marrow megakaryocytes

N-methyl-D-aspartate receptors (NMDARs) are glutamate-gated cation channels with high calcium permeability. Their activity supports megakaryocytes proliferation and maturation, however their downstream effects on the megakaryocyte transcriptome remain poorly elucidated. We used Clariom S Pico Arrays to profile the transcriptome changes caused by loss of Grin1 in mouse bone marrow megakaryocytes. Conditional deletion of Grin1, the gene encoding the obligate NMDAR subunit GluN1, in mouse megakaryocytes was directed by the Pf4 promoter using the Cre-LoxP system. This was achieved by breeding Pf4-Cre with Grin1-loxP/loxP mice. Bone marrow megakaryocytes were isolated by magnetic bead isolation, and total RNA isolated before transcriptome changes were profiled by RNA microarray technology.