Small scale RNAi screen testing juglone and arsenite tolerance.

Genes involved in the C. elegans GSH-metabolism were classified according to their reported or predicted functions. Each of the 67 listed genes was targeted by RNAi starting in L4 animals. F1 L4/young adults of the treated worms were transferred to 0.15 mM juglone and 3 mM arsenite stress plates, respectively, and the survival rates (± SD) were determined after 18 h at 20°C. Significance levels were determined by student’s t-test (n ≥3, at least 90 animals, *PPP+genes possess different isoforms, however, all isoforms are affected by the RNAi constructs used.