Single-cell trajectory analysis of the differentiation of RGCs in the VZ (scRNA-seq)

The largest germinal niche of the adult mammal brain locates at the ventricular zone (VZ), which is made up of adult neural stem cells (NSCs) and multiciliated ependymal cells (EPCs). Both NSCs and EPCs derive from radial glia (RG), whereas the transcriptomic dynamic changes of the cell fate continuum remain elusive. Here, we used single cell RNA-seq of CD133 positive RGCs from the VZ of new born mice to uncover the developmental trajectories of RGCs to NSCs and EPCs.