Crystal structure of the TIR domain from the grapevine disease resistance protein RUN1 in complex with NADP+ and Bis-Tris

Crystal structure of the TIR domain from the grapevine disease resistance protein RUN1 in complex with NADP+ and Bis-Tris Descriptor: 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ADENOSINE-2'-5'-DIPHOSPHATE, TIR-NB-LRR type resistance protein RUN1 Authors: Horsefield, S, Burdett, H, Zhang, X, Manik, M.K, Shi, Y, Chen, J, Tiancong, Q, Gilley, J, Lai, J, Gu, W, Rank, M, Casey, L, Ericsson, D.J, Foley, G, Hughes, R.O, Bosanac, T, von Itzstein, M, Rathjen, J.P, Nanson, J.D, Boden, M, Dry, I.B, Williams, S.J, Staskawicz, B.J, Coleman, M.P, Ve, T, Dodds, P.N, Kobe, B. Deposit date: 2019-02-17 Release date: 2019-09-04 Last modified: 2024-03-13 Method: X-RAY DIFFRACTION (1.75 Å) Cite: NAD+cleavage activity by animal and plant TIR domains in cell death pathways. Science, 365, 2019

本数据集收录葡萄藤抗病蛋白RUN1的TIR结构域（TIR domain）与烟酰胺腺嘌呤二核苷酸磷酸（NADP+）及双(羟甲基)氨基甲烷（Bis-Tris，化学全称：2-[双(2-羟乙基)氨基]-2-羟甲基-丙烷-1,3-二醇）形成复合物的晶体结构。数据集描述：配体包含2-[双(2-羟乙基)氨基]-2-羟甲基-丙烷-1,3-二醇、腺苷-2',5'-二磷酸，以及TIR-NB-LRR型抗病蛋白RUN1（TIR-NB-LRR type resistance protein RUN1）。作者：Horsefield, S.、Burdett, H.、Zhang, X.、Manik, M.K.、Shi, Y.、Chen, J.、Tiancong, Q.、Gilley, J.、Lai, J.、Gu, W.、Rank, M.、Casey, L.、Ericsson, D.J.、Foley, G.、Hughes, R.O.、Bosanac, T.、von Itzstein, M.、Rathjen, J.P.、Nanson, J.D.、Boden, M.、Dry, I.B.、Williams, S.J.、Staskawicz, B.J.、Coleman, M.P.、Ve, T.、Dodds, P.N.、Kobe, B.。沉积日期：2019-02-17；发布日期：2019-09-04；最后修改日期：2024-03-13。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.75埃（1.75 Å）。引用文献：《细胞死亡通路中动植物TIR结构域的NAD+切割活性》，发表于《Science》，2019年，第365卷。