Detection of Phialophora gregata in stems with PCR, observation of hyphae in vessels, and stem symptom severity of resistant and susceptible cultivars at 2 and 6 weeks post inoculation.

aDetection of P. gregata in plants. If P. gregata was not detected using standard PCR, a more sensitive real-time quantitative PCR assay was used.bThe average of the total number of vessels infected with P. gregata in a cross section.cReaction of soybean cultivars to P. gregata; R = resistant, S = susceptible.dPlants inoculated with Type A of P. gregata.ePlants inoculated with Type B of P. gregata.fTreatments were replicated three times (n = 3 plants/replication).gTreatments were replicated three times (n = 2 plants/replication).hTreatments were replicated three times (n = 4 plants/replication).