P. aeruginosa PAO1 wildtype versus PA1226 mutant. P. aeruginosa PAO1 wildtype versus PA1226 mutant

In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in this regulator abolished the ability of P. aeruginosa to produce biofilm in vitro, without any effect on the planktonic growth. This regulator is essential to the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including alginate, pili, and LPS biosynthesis. Overall design: PAO1 wildtype versus PA1226 mutant both grown in LB media. cDNA samples were hybridized onto P. aeruginosa 70mer duplicate array (GEO accession number GPL10633).