Open Reading Frames (ORFs) and annotations from the paired-end transcriptome outputs of deep-sea zoanthid Zibrowius primnoidus RNASeq data
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This dataset contains Open Reading Frames (ORFs) and associated annotations obtained from the paired-end transcriptome outputs of the deep-sea zoanthid Zibrowius primnoidus RNASeq data. The dataset includes the following files: From TransDecoder analyses: • Zibrowius_primnoidus_assembly.Trinity.fasta.transdecoder.cds: Nucleotide sequences for coding regions of the final candidate ORFs, obtained with TransDecoder from the paired-end transcriptome assembly of Zibrowius primnoidus. • Zibrowius_primnoidus_assembly.Trinity.fasta.transdecoder.gff3: Genomic-style coordinates of the final selected ORFs on the Trinity transcripts from the paired-end transcriptome of Zibrowius primnoidus. • Zibrowius_primnoidus_assembly.Trinity.fasta.transdecoder.pep: Peptide sequences for the final candidate ORFs, predicted by TransDecoder from the paired-end transcriptome of Zibrowius primnoidus. • Zibrowius_primnoidus_assembly.Trinity.fasta.transdecoder.bed: BED-formatted file describing ORF positions on the Trinity transcripts, suitable for visualisation in genome browsers such as IGV or GenomeView. From similarity and domain-based annotation: • Uniref90_metazoa.diamond.blastp.outfmt6.w_pct_hit_length: DIAMOND BLASTp results of predicted proteins against the UniRef90 metazoa database, including the top hit length and the percentage of the hit covered by the alignment for each ORF. • pfam.domtblout: Pfam domain annotations for the predicted proteins in the de novo transcriptome assembly of Zibrowius primnoidus. From Trinotate analyses: • Zibrowius_primnoidus_assembly.Trinity.annotation_report.tsv: Comprehensive annotation table generated by Trinotate, including BLAST, Pfam, signal peptide and transmembrane predictions, and other functional annotations for the paired-end de novo assembly of Zibrowius primnoidus. • Zibrowius_primnoidus_assembly.Trinity.annotation_report.html.cXp_summary.html: HTML summary report from Trinotate, providing an overview of the functional annotation and transcript features of the Zibrowius primnoidus assembly.
本数据集收录了源自深海海葵Zibrowius primnoidus的双端转录组RNA测序(RNASeq)数据的开放阅读框(Open Reading Frames, ORFs)及其相关注释信息。数据集包含以下文件: ### 基于TransDecoder分析的文件 • Zibrowius_primnoidus_assembly.Trinity.fasta.transdecoder.cds:从该深海海葵的双端转录组Trinity组装结果中,通过TransDecoder预测得到的最终候选开放阅读框的编码区核苷酸序列。 • Zibrowius_primnoidus_assembly.Trinity.fasta.transdecoder.gff3:记录该深海海葵双端转录组Trinity转录本上最终筛选出的开放阅读框的基因组式坐标信息。 • Zibrowius_primnoidus_assembly.Trinity.fasta.transdecoder.pep:通过TransDecoder从该深海海葵的双端转录组中预测得到的最终候选开放阅读框的肽段序列。 • Zibrowius_primnoidus_assembly.Trinity.fasta.transdecoder.bed:采用BED格式编写的文件,描述了Trinity转录本上的开放阅读框位置,可用于IGV、GenomeView等基因组浏览器进行可视化分析。 ### 基于相似性与结构域注释的文件 • Uniref90_metazoa.diamond.blastp.outfmt6.w_pct_hit_length:将预测得到的蛋白质序列与UniRef90动物界数据库进行DIAMOND BLASTp比对的结果文件,包含每条开放阅读框的最优比对hit长度以及比对覆盖hit的百分比信息。 • pfam.domtblout:针对该深海海葵从头转录组组装结果中预测蛋白质的Pfam结构域注释文件。 ### 基于Trinotate分析的文件 • Zibrowius_primnoidus_assembly.Trinity.annotation_report.tsv:由Trinotate生成的综合注释表格,涵盖了针对该深海海葵双端从头转录组组装结果的BLAST比对、Pfam结构域、信号肽与跨膜结构域预测以及其他功能注释信息。 • Zibrowius_primnoidus_assembly.Trinity.annotation_report.html.cXp_summary.html:Trinotate生成的HTML格式汇总报告,对该深海海葵转录组组装的功能注释与转录本特征进行了整体概述。




