The classical and alternative circulating renin-angiotensin system in normal dogs and dogs with stage B1 and B2 myxomatous mitral valve disease

The behavior of the comprehensive circulating renin‐angiotensin system (RAS) in dogs with myxomatous mitral valve disease (MMVD) before the onset of congestive heart failure remains largely unexplored.
Hypothesis/Objectives: The classical and alternative RAS activity and aldosterone concentrations will be significantly higher in dogs with American College of Veterinary Internal Medicine (ACVIM) stage B2 MMVD compared to normal dogs and dogs with ACVIM stage B1 MMVD.
Animals: One hundred seventeen client‐owned dogs (normal = 60; B1 = 31; B2 = 26).
Methods: Prospective observational study. Angiotensin peptides (AP) and aldosterone concentrations were measured using liquid chromatography and mass spectrometry. Angiotensin converting enzymes 1 and 2 (ACE, ACE2) and renin activity surrogates were calculated from AP concentrations. Equilibrium dialysis (ED) and immediate protease inhibition (PI) methods of AP quantification were compared in 14 healthy dogs.
Results: Core RAS activity and aldo..., Demographic, clinical, and biochemical data were collected prospectively during the clinical visit of each patient.  Data were entered into an Excel spreadsheet by the study investigators.  These data were entered into a commercial statistical software.  The data have not been processed. , , # The classical and alternative circulating renin-angiotensin system in normal dogs and dogs with stage B1 and B2 myxomatous mitral valve disease

[https://doi.org/10.5061/dryad.6wwpzgn66](https://doi.org/10.5061/dryad.6wwpzgn66)

## Description of the data and file structure

**Demographic, physical exam, and biochemical data are included in the files RAAS Project Normal Dogs; RAAS Project B1 Dogs; RAAS Project B2 Dogs**

Tabs: Exam_and_history: demographic, physical exam, and historical information; CBC_Chem: complete blood count and serum biochemistry results; Urine: urinalysis results; Echo: echocardiographic data

Missing data denoted by an empty cell; Data field that are not applicable to a patient are denoted by N/A or n/a; Date: date of exam; Sex: MI=0, MC=1, FI=2, FS=3; Amount: amount of food fed in cups; Na+%: sodium content of diet; MealToCollectionTime: time between meal and blood collection; BP: blood pressure (mmHg); HR: heart rate (bpm); Demeanor: normal=0, nervous or anx...