The classical and alternative circulating renin-angiotensin system in normal dogs and dogs with stage B1 and B2 myxomatous mitral valve disease

The behavior of the comprehensive circulating renin‐angiotensin system (RAS) in dogs with myxomatous mitral valve disease (MMVD) before the onset of congestive heart failure remains largely unexplored. Hypothesis/Objectives: The classical and alternative RAS activity and aldosterone concentrations will be significantly higher in dogs with American College of Veterinary Internal Medicine (ACVIM) stage B2 MMVD compared to normal dogs and dogs with ACVIM stage B1 MMVD. Animals: One hundred seventeen client‐owned dogs (normal = 60; B1 = 31; B2 = 26). Methods: Prospective observational study. Angiotensin peptides (AP) and aldosterone concentrations were measured using liquid chromatography and mass spectrometry. Angiotensin converting enzymes 1 and 2 (ACE, ACE2) and renin activity surrogates were calculated from AP concentrations. Equilibrium dialysis (ED) and immediate protease inhibition (PI) methods of AP quantification were compared in 14 healthy dogs. Results: Core RAS activity and aldo..., Demographic, clinical, and biochemical data were collected prospectively during the clinical visit of each patient. Data were entered into an Excel spreadsheet by the study investigators. These data were entered into a commercial statistical software. The data have not been processed. , , # The classical and alternative circulating renin-angiotensin system in normal dogs and dogs with stage B1 and B2 myxomatous mitral valve disease [https://doi.org/10.5061/dryad.6wwpzgn66](https://doi.org/10.5061/dryad.6wwpzgn66) ## Description of the data and file structure **Demographic, physical exam, and biochemical data are included in the files RAAS Project Normal Dogs; RAAS Project B1 Dogs; RAAS Project B2 Dogs** Tabs: Exam_and_history: demographic, physical exam, and historical information; CBC_Chem: complete blood count and serum biochemistry results; Urine: urinalysis results; Echo: echocardiographic data Missing data denoted by an empty cell; Data field that are not applicable to a patient are denoted by N/A or n/a; Date: date of exam; Sex: MI=0, MC=1, FI=2, FS=3; Amount: amount of food fed in cups; Na+%: sodium content of diet; MealToCollectionTime: time between meal and blood collection; BP: blood pressure (mmHg); HR: heart rate (bpm); Demeanor: normal=0, nervous or anx...充血性心力衰竭发作前，罹患黏液瘤性二尖瓣疾病（myxomatous mitral valve disease, MMVD）犬只的全身性循环肾素-血管紧张素系统（renin-angiotensin system, RAS）活性特征，目前仍未得到充分研究。 研究假设与目标：相较于健康犬及美国兽医内科学会（American College of Veterinary Internal Medicine, ACVIM）分期B1期MMVD患犬，ACVIM分期B2期MMVD患犬的经典与替代通路RAS活性及醛固酮浓度均会显著升高。 实验动物：共117只伴侣犬（健康犬60只，B1期患犬31只，B2期患犬26只）。 研究方法：本研究为前瞻性观察性研究。采用液相色谱-质谱联用法检测血管紧张素肽（angiotensin peptides, AP）及醛固酮浓度；基于AP浓度计算血管紧张素转换酶1、2（ACE、ACE2）活性及肾素活性替代指标。另选取14只健康犬，对比平衡透析（equilibrium dialysis, ED）与即时蛋白酶抑制（protease inhibition, PI）两种AP定量方法的差异。 研究结果：核心RAS活性及醛固酮相关指标…… 本研究前瞻性收集所有受试犬临床就诊时的人口统计学、临床及生化数据，由研究人员录入Excel表格，后续导入商用统计软件进行分析。本数据集尚未经过预处理。 # 健康犬及B1、B2期黏液瘤性二尖瓣疾病患犬的循环经典与替代通路肾素-血管紧张素系统 [https://doi.org/10.5061/dryad.6wwpzgn66](https://doi.org/10.5061/dryad.6wwpzgn66) ## 数据集与文件结构说明 **人口统计学、体格检查及生化数据收录于以下文件：RAAS Project Normal Dogs、RAAS Project B1 Dogs、RAAS Project B2 Dogs** 工作表说明： - Exam_and_history：人口统计学信息、体格检查结果及病史资料 - CBC_Chem：全血细胞计数与血清生化检测结果 - Urine：尿液分析结果 - Echo：超声心动图检测数据 缺失数据以空单元格标注；不适用于受试犬的数据字段以N/A或n/a标注。各字段详细说明如下： - Date：就诊检查日期 - Sex：性别（MI=0：雄性未绝育，MC=1：雄性已绝育，FI=2：雌性未绝育，FS=3：雌性已绝育） - Amount：饲喂食物的杯数 - Na+%：日粮钠含量占比 - MealToCollectionTime：进食至采血的间隔时间 - BP：血压（单位：mmHg） - HR：心率（单位：bpm） - Demeanor：行为状态（normal=0：正常，nervous or anx...：紧张或焦虑……）