Metagenomes from Eastern Brazilian Amazonian floodplains in the wet and dry seasons
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https://www.osti.gov/servlets/purl/1864845/
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资源简介：
Brief sample description<br>Sediment samples from 0 to 10 cm depth were collected in triplicate from two floodplains of the Eastern Brazilian Amazon, one located on the Amazonas River (FP2, 2°28'11.2"S 54°38'49.9"W) and the other at the intersection between the Amazonas and the Tapajós rivers (FP3, 2°22'44.8"S 54°44'21.1"W), in the wet and dry seasons (May and October 2016, respectively). Total DNA was extracted in duplicate from 0.25 g of sediment using PowerLyzer PowerSoil DNA Isolation Kit. The metagenomic libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina and paired-end sequenced (2 x 150 bp) on an Illumina HiSeq 2500 platform. Detailed information about the study sites, sampling, sediment physicochemical properties, DNA extraction and quantification have been previously described by Gontijo et al. (2021).<br>Sample IDs:M1, M2 and M3: FP2, wet seasonM4, M5 and M6: FP3, wet seasonM7, M8 and M9: FP2, dry seasonM10, M11 and M12: FP3, dry season
提供机构：
Center for Nuclear Energy in Agriculture, University of São Paulo, Brazil; Princeton Institute for International and Regional Studies, USA.
创建时间：
2022-04-28
