Genetic variation and hybridization determine the outcomes of conservation reintroductions

The preservation of genetic variation is fundamental in biodiversity conservation, yet its importance for population viability remains contentious. Mixed-source reintroductions, where individuals are translocated into a single vacant habitat from multiple genetically divergent and often depauperate populations, provide an opportunity to evaluate how genetic variation and hybridization influence individual- and relative population fitness. Population genetic theory predicts that individuals with higher genetic variation and hybrids among populations should have higher fitness. We tested these hypotheses by analyzing individual and population-scale data for translocated westslope cutthroat trout (Oncorhynchus clarkii lewisi) and their offspring in four mixed-source reintroductions. We observed more hybrid and fewer nonhybrid offspring than expected across four independent mixed-source reintroductions. We also found clear evidence that heterozygosity predicted individual reproductive- and ..., , , # Genetic variation and hybridization determine the outcomes of conservation reintroductions [https://doi.org/10.5061/dryad.sxksn0380](https://doi.org/10.5061/dryad.sxksn0380) ## Description of the data and file structure These data were collected from several westslope cutthroat trout populations in southwest Montana to better understand native species reintroduction outcomes. Data contain both demographic (length, weight, site collected, population of origin, year sampled, etc.) and genetic data for individuals sampled throughout this study. ### Files and variables #### File: SF_Greenhorn_RAD_Data_NOT_FILTERED.csv **Description:**Â RAD capture sequencing data (genetic data) for donor fish translocated into SF Greenhorn Creek and their offspring sampled following translocation events. This is the unfiltered dataset, meaning no genetic filtering steps (described in the supplemental) have been performed with this dataset. This genetic data set is separate from the other genetic data...,遗传变异的保存是生物多样性保护的核心基础，但其对于种群生存力的重要性仍存在争议。混合来源再引入计划——即从多个遗传分化显著且通常遗传多样性匮乏的种群中选取个体，释放至单一空置栖息地——为评估遗传变异与杂交如何影响个体及相对种群适合度提供了研究契机。种群遗传学理论预测，遗传变异水平更高的个体以及种群间的杂交后代应具备更高的适合度。我们通过分析4项独立混合来源再引入计划中，被移植的西坡割喉鳟（*Oncorhynchus clarkii lewisi*）及其后代的个体与种群尺度数据，对上述假说进行了验证。在4项独立的混合来源再引入计划中，我们观测到的杂交后代数量多于预期，而非杂交后代数量则少于预期。我们还发现明确证据表明，杂合度可预测个体繁殖适合度及…… # 遗传变异与杂交决定保护再引入的结果 https://doi.org/10.5061/dryad.sxksn0380 ## 数据与文件结构说明 本数据集采集自蒙大拿州西南部的多个西坡割喉鳟种群，旨在深入探究本土物种再引入的实施效果。数据集涵盖本研究全程采样个体的人口统计学数据（体长、体重、采样位点、源种群、采样年份等）与遗传数据。 ### 文件与变量 #### 文件：SF_Greenhorn_RAD_Data_NOT_FILTERED.csv **描述：** 用于分析被释放至SF格林霍恩溪的供体鱼类及其移植后采样后代的RAD捕获测序数据（遗传数据）。本数据集为未经过滤版本，即未执行补充材料中所述的任何遗传过滤步骤。该遗传数据集与其他遗传数据集相互独立……