Single cell RNA sequencing of healthy human airway epithelia with IL-13 treatment time course

The goal of this study was to examine changes in cell composition and gene expression with IL-13 treatment over time within different cell types from air-liquid interface cultures from healthy primary human airway epithelia. Well differentiated, primary human airway epithelia at the air liquid interface were dissociated and prepared for single cell sequencing via 10X Genomics Chromium controller, and sequenced on Illumina HiSeq4000