Zebrafish EN/ENCDC RNA-seq

A project to find differential expressed genes between enteric neurons (ENs) and enteric neural crest-derived cells (ENCDCs) in larval zebrafish gut. We dissected guts of transgenic line, Tg(SAGFF(LF)217B; uas:gfp) for ENs and Tg(sox10:cre; EF1alpha:loxP-gfp-loxP-dsred) for ENCDCs, and isolated GFP+ ENs and dsRed+ ENCDCs. Three duplicates for each of ENs and ENCDCs are prepared. Libraries for NGS are prepared using SMART-Seq_V4 Ultra Low Input RNA Kit.