CRISPR_SAM_screens. CRISPR_SAM_screens

CRSPR SAM libraries will be used in cancer cell lines to identify resistance mechanisms to anti-cancer drugs. Cancer cell lines will be given a whole-genome libraries, in order to create a frmaeshifts in the cell population. After cells will be exposed to anti-cancer drugs and resistant cells will be collected for DNA extractions and future sequencing. Amplicons containing gRNA sequences will be amplified , librarie will be created with different tags for Illumina sequencing.