Prostate H3K4me3 ChIP-seq

Exposure to pesticide chlordecone is associated with high risk of prostate cancer.We hypothesised that changes in histone H3K4me3 could be involved in pathological processes in prostate induced by chlordecone. We exposed mice to chlordecone during embryonic development (E6.5-E15.5) and we analysed F1 progeny male epigenetic H3K4me3 marks in prostate tissue, as well as H3K4me3 in the prostate of F3 progeny to reveal the transgenerational effects resulting from the ancestral exposure to CD. We performed ChIP using H3K4me3 commercial antibody (Merck Millipore, 07-473) and pooled prostate tissues from 4 mice. DNA-protein complex was immunoprecipitated, eluted and DNA purified. Overall design: The DNA from ChIP, samples of F1 animals: 1. PS1 control 2. PS2 control 3. PS3 chlordecone 4. PS4 chlordecone 5. PS5 input Four libraries (H3K4me3 ChIP) were prepared from prostate of F3 mice. Two replicates from control-derived mice (C5, C7) and two replicates from CD-derived mice (K4, K11).