SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in March 2018

Flow cytometry data was collected in March 2018, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.本数据集的流式细胞术（Flow Cytometry）数据采集于2018年3月，采集地点为澳大利亚南部近海海域。本研究的总体目标为：建立澳大利亚南部大陆架海域生态系统的背景知识体系，阐明上升流/高盐溢流出事件对微生物群落的影响，最终构建该区域的生物地球化学模型。 采样工作作为澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目的组成部分，在RV Ngerin号科考船的巡航任务中开展。每次巡航期间，研究人员均对叶绿素荧光最大值（Deep Chlorophyll Maximum, DCM）层的物理、化学及生物学特性开展调查。本次研究已针对超微型浮游植物、细菌与病毒采集了流式细胞术数据。 本研究共布设并采样了6个主要站位：其中5个站位位于100米等深线处，依次为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）及SAM5CB/B5（35.008°S，135.198°E）；剩余1个为位于袋鼠岛西南侧的远海站位B1（36.188°S，136.178°E）。需说明的是：结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）以及科考船的平均航速（即9节），可证实各站位所采集的水样分属不同水团。 此外，研究人员偶尔还会在袋鼠岛国家参考站（National Reference Station, NRS，35.832°S，136.447°E）以及发生高盐溢流出的SA斯潘塞湾口锚泊站（SAM8SG，35.25°S，136.690°E）采集补充样本。