DNA regions bound by Myxococcus xanthus ParB and FrzCD

Our ChipSeq analysis show that while FrzCD does not bind DNA specific regions, ParB binds parS consistent with what as been previously shown. The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. FrzCD directly binds to the nucleoid and the FrzCD binding to the DNA leads to the formation of chemosensory complexes. This supra-molecular organization is required for cooperative interactions between clustered receptors, in turn important for the modulation of bacterial social behaviors. Overall design: ChIp-seq was performed as previously described (Fioravanti et al., 2013). Cells were grown and then fixed with formaldehyde as previously described (Fioravanti et al., 2013).