Expression analysis of microRNA in malignant peritoneal mesothelioma induced by intraperitoneal injections of iron saccarate in rats

Iron plays an important role in oxidative tissue damage and subsequent carcinogenesis. Iron deposition in the peritoneum causes neoplastic changes. Here we used microRNA (miR) microarrays in a rat model by repeated intraperitoneal injections of ferric saccharate. miR microarray revealed the miR-199/214 as a distinctive feature of sarcomatoid mesothelioma. Carcinogenesis protocol was performed using specific pathogen-free male and female F1 hybrid rats between Fischer344 and Brown-Norway strains. A total of 8 samples were analyzed by microRNA (miR) microarray for the screening purpose. Eight samples follows; the immortalized rat peritoneal mesothelial cell and mesothelial tissue collection as non-neoplastic mesothelia;iron saccharete-Induced four cases of epithelioid mesothelioma and two cases of sarcomatoid subtypes. The six mesothelioma samples were obtained from the same tumors also analyzed in our previously submitted experiment (GSE16138).