Artificial mixtures of different heteroplasmy frequencies.

Two rhesus macaque samples differing in 14 fixed sites, and two heteroplasmic sites (one in each macaque; shown in green) were mixed at different ratios (10%, two replicates; 1%, two replicates; and 0.2%, six replicates). The mean and STDEV across all sites were calculated without the heteroplasmic sites. Nineteen out of 168 heteroplasmic sites at a mixed frequency of about 0.2% could not be detected in DCSs (MAFs) shown in red), but all of them were present in SSCSs. DCS, duplex consensus sequence MAF, minor allele frequency; SSCS, single-strand consensus sequence; STDEV, standard deviation. (XLSX)