First evidence of WW superfemale for hybrid sturgeon, the bester (Huso huso × Acipenser ruthenus)

Sex control is a promising approach for cost-effective aquaculture in certain fish species. Sturgeons possess ZZ/ZW genetic sex determination system and are popular aquaculture target for high-priced black caviar originated from female roe. Therefore, the production of all-female population is highly desired, but this has not yet been achieved to date. In this study, we identified a short nucleotide sequence specific to putative Z and W scaffolds of Acipenser ruthenus genome and developed the PCR to discriminate Z and W alleles. Using this PCR, A. ruthenus (N = 1,280), A. baerii baerii (N = 66), and F2 of the bester hybrid (Huso huso × A. ruthenus: N = 31) were segregated into dimorphic pattern of a single Z amplicon and dual Z plus W amplicons, with approximately 1:1 ratio. Of those, the gonadal sexes were identified for 145 individuals, and all were concordant with their genetic sex. Additionally, we applied the Z/W-discriminative PCR for gynogenetic population of F3 bester and identi..., , , # Data from: First evidence of WW superfemale for hybrid sturgeon, the bester (Huso huso × Acipenser ruthenus)

[https://doi.org/10.5061/dryad.msbcc2g1v](https://doi.org/10.5061/dryad.msbcc2g1v)

In this study, we developed a PCR to discriminate Z/W sex chromosome for sturgeons.

This dataset contains the individual results of genetic and gonadal sexing for three sturgeon spesies.

## Description of the data and file structure

DATA & FILE OVERVIEW

This dataset contains thee CSV files:

genetic_and_gonadal_sexes_of_cultured_sturgeons.csv, individual data collected from the aquacultured sturgeons focusing on their genetic and gonadal sexes;

gonadal_sexes_of_dead_Acipenser_ruthenus.csv, individual data collected from previously-genotyped Acipenser ruthenus and necropsied after death for gonadal sexing;

genetic_sexes_of_chromosome_manipulated_bester.csv, individual data collected from chromosome-manipulated bester genotyped for comparison between experimental groups

METHODOLOGICAL ...