First evidence of WW superfemale for hybrid sturgeon, the bester (Huso huso × Acipenser ruthenus)

Sex control is a promising approach for cost-effective aquaculture in certain fish species. Sturgeons possess ZZ/ZW genetic sex determination system and are popular aquaculture target for high-priced black caviar originated from female roe. Therefore, the production of all-female population is highly desired, but this has not yet been achieved to date. In this study, we identified a short nucleotide sequence specific to putative Z and W scaffolds of Acipenser ruthenus genome and developed the PCR to discriminate Z and W alleles. Using this PCR, A. ruthenus (N = 1,280), A. baerii baerii (N = 66), and F2 of the bester hybrid (Huso huso × A. ruthenus: N = 31) were segregated into dimorphic pattern of a single Z amplicon and dual Z plus W amplicons, with approximately 1:1 ratio. Of those, the gonadal sexes were identified for 145 individuals, and all were concordant with their genetic sex. Additionally, we applied the Z/W-discriminative PCR for gynogenetic population of F3 bester and identi..., , , # Data from: First evidence of WW superfemale for hybrid sturgeon, the bester (Huso huso × Acipenser ruthenus) [https://doi.org/10.5061/dryad.msbcc2g1v](https://doi.org/10.5061/dryad.msbcc2g1v) In this study, we developed a PCR to discriminate Z/W sex chromosome for sturgeons. This dataset contains the individual results of genetic and gonadal sexing for three sturgeon spesies. ## Description of the data and file structure DATA & FILE OVERVIEW This dataset contains thee CSV files: genetic_and_gonadal_sexes_of_cultured_sturgeons.csv, individual data collected from the aquacultured sturgeons focusing on their genetic and gonadal sexes; gonadal_sexes_of_dead_Acipenser_ruthenus.csv, individual data collected from previously-genotyped Acipenser ruthenus and necropsied after death for gonadal sexing; genetic_sexes_of_chromosome_manipulated_bester.csv, individual data collected from chromosome-manipulated bester genotyped for comparison between experimental groups METHODOLOGICAL ...性别调控是部分鱼类实现低成本水产养殖的极具前景的策略。鲟鱼拥有ZZ/ZW型遗传性别决定系统，且因其雌性鱼卵可产出高价黑鲟鱼子酱而成为热门的水产养殖对象。因此，全雌种群的生产具有极高的应用价值，但目前尚未实现。 本研究中，我们鉴定出俄罗斯鲟（Acipenser ruthenus）基因组中推定的Z、W染色体支架特异性短核苷酸序列，并开发了可区分Z、W等位基因的聚合酶链式反应(Polymerase Chain Reaction, PCR)技术。利用该PCR技术，我们对1280尾俄罗斯鲟、66尾西伯利亚鲟指名亚种（A. baerii baerii）以及31尾鲟鳇杂交F2代（欧洲鳇Huso huso × 俄罗斯鲟A. ruthenus）进行基因分型，结果均呈现出单Z扩增产物与Z+W双扩增产物的二态性模式，且二者比例约为1:1。其中，我们对145尾个体进行了性腺性别鉴定，所有个体的遗传性别与性腺性别均保持一致。此外，我们将该Z/W鉴别PCR技术应用于鲟鳇杂交F3代的雌核发育种群，并…… 【数据来源：杂交鲟鳇（欧洲鳇Huso huso × 俄罗斯鲟Acipenser ruthenus）WW超雌个体的首份证据】 https://doi.org/10.5061/dryad.msbcc2g1v 本研究开发了可区分鲟鱼Z/W性染色体的PCR技术。 本数据集包含三种鲟鱼的遗传性别与性腺性别鉴定的个体数据。 ## 数据与文件结构说明 ### 数据与文件概览 本数据集包含3个CSV格式文件： 1. genetic_and_gonadal_sexes_of_cultured_sturgeons.csv：收录自养殖鲟鱼的个体数据，聚焦其遗传性别与性腺性别信息； 2. gonadal_sexes_of_dead_Acipenser_ruthenus.csv：收录自已完成基因分型的俄罗斯鲟个体数据，这些个体在死后通过剖检进行性腺性别鉴定； 3. genetic_sexes_of_chromosome_manipulated_bester.csv：收录自经染色体操作的鲟鳇杂交种个体数据，对其进行基因分型以用于实验组间的比较分析。 方法学部分……