Phenols HPLC

To investigate the potential role of photoprotective and antioxidant mechanisms in response to future increases in sea surface temperatures caused by climate change, we conducted laboratory experiments using three intertidal macroalgae model species: Adenocystis utricularis (Ochrophyta, Phaeophyceae), Pyropia endiviifolia (Rhodophyta, Bangiophyceae), and Monostroma hariotii (Chlorophyta, Ulvophyceae). These algae were collected in Punta Artigas (King George Island, Antarctica) and acclimated at 2°C for 48 hours. They were then assessed in laboratory experiments for up to 5 days under two treatments: 1) control conditions at 2°C, and 2) elevated temperature conditions at 8°C, representing the most negative increment in SSTs estimated by the end of the 21st century. Pigments (chlorophylls and carotenoids), Mycosporine-like amino acids (MAAs), and Phenolic compounds were quantified after 3 and 5 days of exposure.为探究光保护与抗氧化机制在应对气候变化引发的未来海表温度升高中的潜在作用，我们采用三种潮间带大型藻类模式物种开展了室内实验：囊腺藻（Adenocystis utricularis，Ochrophyta，Phaeophyceae）、安德维夫紫菜（Pyropia endiviifolia，Rhodophyta，Bangiophyceae）、哈氏单条藻（Monostroma hariotii，Chlorophyta，Ulvophyceae）。上述藻类采集自南极洲乔治王岛的阿蒂加斯角（Punta Artigas），并在2℃条件下驯化48小时。随后我们设置两组处理开展室内实验，对受试藻类进行最长5天的评估：1）对照组：2℃恒温环境；2）升温处理组：8℃恒温环境，该升温幅度对应21世纪末海表温度（sea surface temperatures, SSTs）预估的最极端增量。在暴露3天与5天后，我们对色素（叶绿素与类胡萝卜素）、类菌孢素氨基酸（Mycosporine-like amino acids, MAAs）以及酚类化合物进行了定量分析。