X-ray crystal structure of UDP- 2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27 in complex with its product UDP-2,3-diacetamido-2,3-dideoxy-d-mannuronic acid at pH 9

X-ray crystal structure of UDP- 2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27 in complex with its product UDP-2,3-diacetamido-2,3-dideoxy-d-mannuronic acid at pH 9 Descriptor: (2~{S},3~{S},4~{R},5~{S},6~{R})-4,5-diacetamido-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxane-2-carboxylic acid, 1,2-ETHANEDIOL, CHLORIDE ION, ... Authors: McKnight, J.O, Thoden, J.B, Holden, H.M. Deposit date: 2023-05-24 Release date: 2023-09-13 Last modified: 2023-10-18 Method: X-RAY DIFFRACTION (2.1 Å) Cite: Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis. J.Biol.Chem., 299, 2023嗜热栖热菌（Thermus thermophilus）菌株HB27来源的UDP-2,3-二乙酰胺基-2,3-二脱氧葡糖醛酸-2-表异构酶（UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase）的X射线晶体结构（X-ray crystal structure），该复合物在pH 9条件下与其产物UDP-2,3-二乙酰胺基-2,3-二脱氧-d-甘露糖醛酸（UDP-2,3-diacetamido-2,3-dideoxy-d-mannuronic acid）结合。 描述符：(2S,3S,4R,5S,6R)-4,5-二乙酰胺基-6-[[[(2R,3S,4R,5R)-5-[2,4-二氧代嘧啶-1-基]-3,4-二羟基四氢呋喃-2-基]甲氧基-氧代磷酰基]氧基-氧代磷酰基]氧基-3-羟基四氢吡喃-2-羧酸、1,2-乙二醇（1,2-ETHANEDIOL）、氯离子（CHLORIDE ION）…… 作者：McKnight, J.O.、Thoden, J.B.、Holden, H.M. 沉积日期：2023-05-24 发布日期：2023-09-13 最后修改日期：2023-10-18 实验方法：X射线衍射（X-RAY DIFFRACTION，分辨率2.1埃（2.1 Å）） 引用文献：《细菌UDP-糖2-表异构酶的结构分析揭示催化前后的活性位点构象》，《生物化学杂志（J. Biol. Chem.）》，第299卷，2023年