missing proteins

Chromosome-Centric Human Proteome Project (C-HPP) was initiated in 2012, which aims at discovery of all the proteins encoded by human genome in all the individual chromosomes. The primary goal of C-HPP is to ensure whether all the gene annotations in the human genome could be identified at protein level. If some proteins encoded by certain genes are lack of any experimental evidence, they are termed as missing protein (MP). With fast development of proteomic technology, the scale of MPs has been shrinking continuously, and approximately 1,470 MPs have remained to be explored. It is a key question in this field how to discover the remaining MPs with new technology or analytical strategy. The previous reports have unraveled that in aneuploidy cell lines, the changes of chromosomes, more or less, proportionally matched with the abundance alternations in the transcriptome and proteome of the corresponding genes in the aneuploid chromosome(s). Herein, we hypothesize that a stable aneuploid cell line with increasing chromosomes was a useful material that assists the MPs discovery.