Hamm and Gray 2024 Figure 5

FIG 5 Strains lacking polyP and (p)ppGpp have disrupted cell division. (A) Confocal fluorescence time-lapse microscopy of the mutant ppk relA spoT FtsZ-GFP (MJG2405) on an LB agarose pad at 37°C. The triple mutant forms two Z-rings in the middle of the cell, releasing a mini cell (red arrows). There are also two Z-rings which form at either pole of a single cell, both functional and releasing a mini cell (white arrows). Example of mini-cell formation and release can be seen in CEM (S4). (B) Confocal fluorescence time-lapse microscopy of the mutant ppk relA FtsZ-GFP (MJG2403) on an LB agarose pad at 37°C. This image shows a mutant forming three Z-rings at one pole, and at least 3 at the opposite pole as well, with no Z-rings forming in the middle of the cell, for a total of six Z-rings in a single cell. This cell continued to grow without lysing (Supplemental Video SV5).