Enzymatic cascades for steroid glucosylations

<p>In the “2025_steroid_glucosylation” folder is available file with supplementary materials for the publication. </p><p>In the “Colorometric test” folder available are the measurements of pNP absorbance from the reusability test of the co-immobilized enzymes.</p><p>In the “UPLC analysis” folder chromatograms are available depicting substrate conversions in all performed test including: substrate scope investigation, temperature and pH optimization, thermal stability, immobilization resins test, preparative reactions, in vivo reactions, storage test.</p><p>In the &#34;NMR analysis&#34; folder, NMR spectra of isolated/purified biocatalysis products are provided.</p><p>In the “Plasmids and sequences” folder are available all plasmids maps used in the study and query and output protein sequences attained during the search of new GTs.</p><p>In the “Protein analysis” folder are available SDS-PAGE gels photos and data from Bradford analysis.</p><p>In the “Structures and docking” folder are available two folders: “Protein models” folder that contains PDB files of AlphaFold models of selected unidentified GT. In ‘Docking models” are available 16 folders, each dedicated to one protein and each containing .conf, .pdb and .pdbqt files attained during docking experiments.</p>